vstars Chapter 245 Penicillin Sodium!
Chapter 245 Penicillin Sodium!
How to find a melon with green mold in the cold and dry Jin area in late autumn was a headache for Zhao Gang.
melon?
And it has to be a melon with green mold?
Zhao Gang searched almost the entire base, and found that there were indeed melons in the cellar, and he could find quite a few by gathering together the entire base.
But none of them are moldy.
This made Zhao Gang very worried. What the hell should he do?
In the end, it was Sister Xiuqin who really helped a lot.
"Political Commissar, I heard that you have been looking for things with green mold. Can you see if this paste from our textile factory can be used?"
Xiuqin came to Zhao Gang holding a bowl of paste that had turned into green mold.
"Hmm? Sister-in-law, how come your paste is moldy in such cold weather?"
"We had just spun the cotton cloth needed by the team a while ago, and we led the female workers in the textile factory to work overtime, burning the stove day and night.
The whole factory was warm, and a lot of paste had grown mold. We had thrown away a lot of it before. "
Xiuqin handed the bowl of paste to Zhao Gang, and Zhao Gang almost cried. He had been searching for it everywhere but never expected to find it right under his nose.
At the beginning, Yu Ye told him that he could look for Xiuqin, but Zhao Gang didn't take it seriously. Unexpectedly, he really got the green moldy paste from Xiuqin in the end.
Yu Ye was also very excited when he heard the news. He didn't expect that he could actually find this Penicillium. Contrary to the general impression, Penicillium only has a clear color, not just pure green.
Yu Ye could be sure that this large mass must contain the original strain of Penicillium that he needed.
However, the penicillin content produced by this natural penicillin strain can only be said to be negligible. If these Penicillium fungi are really used as strains, even if Yu Ye is worked to death, he will definitely not be able to extract enough penicillin for a wounded soldier.
Because the penicillin content in this original Penicillium is so low, working for a long time may not be useful.
"Sister-in-law, please ask the aunts to cook some fine corn porridge. It must be boiled for a long time. Also, give me some small pots and boil water for these pots.
Bring some vegetable oil and vinegar."
Yu Ye said to Xiu Qin.
"Okay, I'll do it right away."
Although no one knew what Yu Ye was going to do, the prestige he had accumulated over the past year meant that no one would ask why Yu Ye did this and they would just do what Yu Ye said.
Soon, under Yu Ye's operation, the entire textile factory was filled with sterilized jars of various sizes, all of which used corn milk as the culture medium, in which these bacteria grew wildly.
The batch of melons that Zhao Gang got at the beginning were not wasted. They were placed in the house next to the textile factory. Yu Ye also placed a stove in it to simply control the indoor temperature.
In these rooms where there are not even thermometers, green mycelium is growing wildly.
Looking at the green mold growing in several rooms, Yu Ye suddenly had a sense of harvest.
While the mycelium was growing, Yu Ye was not idle either. He began to prepare some reagents using various glass instruments that he had purchased over the past year.
"Director, you took all my equipment without even telling me. Is that really okay? I haven't even finished my RDX industrial scale-up experiment yet."
Chen Kan was very angry about Ye taking away his entire set of glass instruments.
"What do you mean by your equipment? Didn't I buy the instrument in this factory for you?" Yu Ye was concentrating on preparing the saturated ethanol solution of sodium acetate without raising his head.
"I......"
Chen Kan was choked.
Because he found that he had no way to refute what the factory director said. All these instruments were purchased by Yu Ye through various channels after he received funding from various campaigns and headquarters.
Buy ten test tubes today, three beakers tomorrow, and build up this family business bit by bit.
Due to the Japanese blockade, purchasing these equipment was not so easy.
"Calm down, calm down. Just help me first, and I'll write you the preparation route of the all-nitrogen anion salt later."
Yu Ye's light words made Chen Kan smile happily.
Chen Kan even ran up to Yu Ye in an unusually servile manner and said, "Director, is there anything else I can do for you? You don't need to do such rough work. I will definitely do it beautifully for you."
"What? With the hope of all-nitrogen anion salt, RDX is no longer attractive to you?" Yu Ye said unhappily.
"No, no, for those of us who deal in explosives, the stronger the explosive power, the better we like it."
Chen Kan picked up the tools that Yu Ye placed on the table and continued to help Yu Ye distill ethanol.
"Okay, we have already prepared the sodium acetate ethanol solution. You don't need to prepare it anymore. Just pour out half of the culture medium in those jars and put them together.
Our extraction work is about to begin."
"Okay, I'll do it right away."
Chen Kan's eyes lit up. He had heard that the factory director was producing a powerful antibacterial drug that was even more powerful than sulfonamide. He didn't expect to find something hot here.
Fighting back his nausea, Chen Kan collected a bucket of culture medium and placed it in front of Yu Ye.
Yu Ye poured a pound of vegetable oil into it and asked Chen Kan to stir it constantly. The smell was still a bit unpleasant, and Chen Kan rolled his eyes several times.
"Okay, now that you've mixed it, put it aside and let it sit for a few days."
As he spoke, Yu Ye took out a culture medium that had already completed static stratification from under the operating table, and began to pour out the clear liquid at the bottom in front of Chen Kan.
"I......"
Chen Kan was stunned when he saw the factory manager's action.
"Director, you've already mixed one bucket, why do you want me to mix another bucket? This smell is not good."
As he spoke, Chen Kan dry-heaved twice.
"If I don't allow you to stir it, do you still want me to stir it?"
Yu Ye made a simple mask with cotton cloth mixed with charcoal and put it on under Chen Kan's astonished eyes.
"Director, you are too unkind, aren't you? You have something like this, but you won't give it to me?"
Chen Kan's face was filled with grief and indignation.
"Watch out, kid. Remember every word I say next. This is Nobel Prize-level achievement and experience."
"Tong Zhou, put down the notebook in your hand and watch my movements carefully. From now on, all of these will be handled by you."
The young man who studied biology had been following Yu Ye since the very beginning of preparing the fungus strains. He was very silent, but he wrote down every step of Yu Ye's work firmly in his notebook and in his heart.
Chen Kan and Tong Zhou stared at Yu Ye's every move with wide eyes.
"Penicillin is a water-soluble substance, so the lower clear liquid contains penicillin. We then use carbon powder washed with distilled water to filter the clear liquid.
The clear liquid after filtration contains penicillin.
Then we use a saturated ethanol solution of sodium acetate to react with the clear solution, and finally concentrate the crystals to get penicillin sodium crystals. "